Additionally, phenol red tubes contain an inverted Durham tube. Phenol Red Indicator Structure Search; Print SDS. Previous. The pH of blood ranges from 7.35 to 7.45, for instance. 59 0 obj
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The phenol red indicator is discussed. The pH of blood ranges from 7.35 to 7.45, for instance. 0
Phenol red exists as a red crystal that is stable in air. Phenol Red sodium salt, ACS reagent. A solution of phenol red is used as a pH indicator: its color I. '�.A��� ��m�B��-� p�����y��m@x�� ��3�*J>��#��F�D>R�)�@R��(�1� �#ʹR��RC0�7a�����D1��Q� �\S �IJ"7�sを�����\��D����f��w��$��W'��9���;�?�Ҿ�儝�)Z9���CD��� u�z�aXHH^� � Ԏ�e��6v��w8�.�R�h�I��%5� �Xh��G ����X�fcу�X 7�{=�ޛ���A�B ����+��oڑ�B@����HO���.���~�ٴ#��~��.�I�%¢����'N�䃂H;�v�wi�'�{IZ��B@�i=�큥٠�(y����G;�|�!=a@H�f�. Depending on the intended cell type different mixtures of salts, amino acids, vitamins, buffers, carbohydrates, fatty acids, and supplements such as serum are used. This usually results in an acidification of the medium, turning it yellow. Empirical Formula (Hill Notation) C 19 H 14 O 5 S . Formula: C₁₉H₁₄O₅S MW: 354.38 g/mol Storage Temperature: Ambient: MDL Number: MFCD00003552 CAS Number: 143-74-8: Order Now. ���ɨ#�3#P[ƶ�.K�-CV�3v$N$p B�@�] �e8: �yP;P��C�Q?y��P�1 ����o�z�iЯ.����y�`�,�8{�m-b��yR!�7��
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"Y�"���`�"0��^ �"�R@$W&X6,k fk�H�H)} DSSTox_GSID_44420. Chemical Formula: C 19 H 14 O 5 S Brand: OXFORD [9] It was the first test of kidney function and was used for almost a century but is now obsolete. [1][7] However, this cyclic structure could not be confirmed by X-ray crystallography. Molecular Weight 354.38 . The test is based on the fact that phenol red is excreted almost entirely in the urine. h��[k����+��F"l�M�=,Kε�H���6��Y`�5�u�����T�=�=3;;�R^��f�XUdUž�h:Ӈ.����E��/ߙ������r����d��+(�}��KΡK��>�.Y��"�.�T����R�P
�Cz���DH]ʜ�Gm�GS��#�3z��R`�JE�cJ}� L���&c&l�2zN�!���z�}�]A��t=g���\ Phenol red salt is used routinely as pH indicator. Phenol red is a weak estrogen mimic, and in cell cultures can enhance the growth of cells that express the estrogen receptor. Find chemicals for your experiments at Home Science Tools! In crystalline form, and in solution under very ac… Standardized for colorimetric pH determination. Bottle/50 mL spec. MDL number MFCD00003552 February 28, 2019 admin Ingredients 0. Color range: pH 6.0-8.5. 126 0 obj
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Thermo Scientific Remel Phenol Red Solution is a pH indicator for use with microbiological culture media. Phenolsulfonphthalein is being investigated for use clinically due to its weak estrogen mimicking actions. High levels of bromine can convert phenol red to bromophenol red (dibromophenolsulfonephthalein, whose lowered pKa results in an indicator with a range shifted in the acidic direction – water at pH 6.8 will appear to test at 7.5). 2. It is yellow below 6.8 pH and bright fushia (pink) above 8.2 pH. Acid production: 1. endstream
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pH indicators generally change color over a range of two pH units. Phenol red is frequently used in cell culture media as a pH indicator. A small amount of phenol red added to this growth medium will have a pink-red color under normal conditions. The transition range may shift slightly depending on the concentration of the indicator in the solution and on the temperature at which it is used. There are numerous formulations for in vitro cell propagation. Polio (IPV – Ipol) Next. DSSTox_RID_80214. Bovine Brain Endothelial Cell Growth Medium without Phenol Red (500 ml) 1 Product Result �g���_p�����zq���D_�2������{�������볷W�W�}~������W�~����˿�}~���_~�8{�⮗����خ��O�8�� @N���/O�������=����?�������Y �:��l��ǧo�/~����o�g�. In the event of problems, waste products produced by dying cells or overgrowth of contaminants will cause a change in pH, leading to a change in indicator color. [13], Chlorine can result in the bleaching of the dye in the absence of thiosulfate to inhibit the oxidizing chlorine. Many of these defined mixtures also contain phenol red.
Indicators usually exhibit intermediate colors at pH values inside the listed transition range. Add all three to Cart Add all three to List. For example, a culture of relatively slowly dividing mammalian cells can be quickly overgrown by bacterial contamination. Albert P Li. DTXSID2044420. Calculations like this one might make a good project for a college freshman chemistry class. Bottle/50 mL spec. A solution of phenol red is used as a pH indicator, often in cell culture. Phenolsulfonphthalein or otherwise called phenol red is a pH indicator commonly used in cell biology laboratories. They are used to visually signal the acidity or alkalinity of an aqueous (water-based) solution. Negative:The tube containing medium will remain red, indicating the bacteria cannot ferment that particular carbohydrate source present in the media.II. Cite. Phenol Red Indicator, ACS reagent, CAS Number: 143-74-8,(32661 Fluka).Shop now or request a quote. Phenol red (also known as phenolsulfonphthalein or PSP) is a pH indicator frequently used in cell biology laboratories. Above pH 8.1, phenol red turns a bright pink (fuchsia) color. HY-D0169A. grade. Its solubility is 0.77 grams per liter (g/L) in water and 2.9 g/L in ethanol. Phenol red SDS Safety Data Sheet for Phenol red 107241. Since the color of phenol red can interfere with some spectrophotometric and fluorescent assays, many types of tissue culture media are also available without phenol red. phenol red indicator. %PDF-1.6
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Phenol red is yellow at a pH < 6.8 and red at a pH of > 7.4, therefore if a bacterium ferments a sugar to acid a yellow color will develop. Phenol red is a pH indicator frequently used in cell biology laboratories. It indicates that there is drop in the pH because of the production of the acid by the fermentation of the carbohydrate (sugar) present in the media. SCHEMBL222482. Typically, 15 mg/l are used for cell culture. It is a weak acid with pK a = 8.00 at 20 °C (68 °F). By measuring the amount of phenol red excreted colorimetrically, kidney function can be determined.[10]. h�b``�a``�b 'P�0p4p ��@1C � �(��u �8�z0E00�1�2:+0(�{0�0IU1�jPO`d�R`�a���ʰ����QoC��5�8y��[)p]`�����ɰ��"%�Кp;��?�i��H�c�; e�bVf[(�@� Р!#
Its color exhibits a gradual transition from yellow (λmax = 443 nm[2]) to red (λmax = 570 nm[3]) over the pH range 6.8 to 8.2. Above pH 8.2, phenol red turns a bright pink (fuchsia) color. Color range: pH 6.0-8.5. Phenol red exists as a red crystal that is stable in air. When cells are grown in tissue culture, the medium in which they grow is held close to this physiological pH. Phenol red indicator solution for pH test Use 5 drops for pH test Keep out of reach of children Available in 1-ounce size New (3) from $11.99 + FREE Shipping. grade. It is made by condensing two moles of phenol with one mole of o-sulfobenzoic acid anhydride. Even higher levels of bromine (>20 ppm) can result in the secondary conversion of bromophenol red to bromophenol blue with an even lower pKa, erroneously giving the impression that the water has an extremely high pH despite being dangerously low. A solution of phenol red will have a yellow color at a pH of 6.4 or below and a red color at a pH… If the organism is able to utilize the carbohydrate, an acid by-product is created, which turns the media yellow. use of color change of phenol red as the indicator in titrating poliomyelitis virus or its antibody in a tissue–culture system 1 2 This form is sometimes symbolically written as H+2PS− and is orange-red. (A table of other common chemical indicators is available in the article on pH indicators.). MW 354.37. pH indicator widely used in culture media to identify pH changes from neutral (red) to acidic (yellow) values. Phenol red exists as a red crystal that is stable in air. [6], In several sources, the structure of phenol red is shown with the sulfur atom being part of a cyclic group, similar to the structure of phenolphthalein. More information about phenol red indicator in vaccines coming soon. %%EOF
Phenol red solution is administered intravenously, the urine produced is collected. Many biologists find this a convenient way to rapidly check on the health of tissue cultures. Specification Test Results. ?���3��.�ߞ�9;����?���VF�{�����Ƌ�wgW?�����woN/$������ӫӋ������O^\������o��IU����/W��N~���.�� CV�?�1��ɫ���!��D\ͣ�@�pz�H9����'�s����i pH Indicator Ranges (Ascending pH) Acid-base pH indicators are solutions that have a characteristic color at certain pH levels. Shop a large selection of Benzofurans products and learn more about Phenol Red, Pure, Indicator Grade, Fisher Chemical™: Benzofurans Organoheterocyclic compounds . Adenovirus Anthrax (Biothrax) BCG (Tice) Cholera (Vaxchora) DT (Sanofi) DTaP (Daptacel) Phenol Red, sodium salt. A small amount of phenol red added to this growth medium will have a pink-red color under normal conditions. [8], Several indicators share a similar structure to phenol red, including bromothymol blue, thymol blue, bromocresol purple, thymolphthalein, and phenolphthalein. Phenol red has been used as a pH indicator dye in tissue culture media for decades (Figure 1). [4][5], In crystalline form, and in solution under very acidic conditions (low pH), the compound exists as a zwitterion as in the structure shown above, with the sulfate group negatively charged, and the ketone group carrying an additional proton. Phenol Red is used as a pH indicator in cell culture applications. [12], Phenol red, sometimes labelled with a different name, such as "Guardex Solution #2", is used as a pH indicator in home swimming pool test kits. 3 The two forms of the dye have different optical absorption spectra. Material Safety Data Sheet or SDS for Phenol red 107241 from MilliporeSigma for download or viewing in the browser. 13 0 obj
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Hence, the relative concentration of one form, which varies as a function of … Beilstein/REAXYS Number 326470 . Principle: Phenol Red Fermentation medium contains peptone, phenol red (a pH indicator) and the carbohydrate to be tested. It is a weak acid with pKa= 8.00 at 20°C. It is provided as an optional media component when preparing complete media as part of a complete ATCC … Typically a change in color from red to yellow indicates high rates of… Phenol red, pH indicator (CAS 143-74-8) (ab146336) | Abcam [1] It is a weak acid with pKa = 8.00 at 20 °C (68 °F). Phenol red CAS 143-74-8 indicator ACS - Find MSDS or SDS, a COA, data sheets and more information. Frequently bought together + + Total price: $29.48. That I am using is " Phenol Red indicator, ACS reagent, CAS Number 143-74-8 ". These items are shipped from and sold by different sellers. 16th Jan, 2015. Phenol Red Indicator Solution: Dissolve 0.1 g of phenol red in 2.82 ml of 0.1 M sodium hydroxide and 20 ml of ethanol (95 percent). Phenol red is a pH indicator which turns yellow below a pH of 6.8 and fuchsia above a pH of 7.4. Standardized for colorimetric pH determination. Gas Production 1. Phenol red is an indicator commonly used to measure pH in swimming pool test kits, see e.g. endstream
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The phenol red dye indicator is a weak organic acid, and its unionized acid and base forms are present in a concentration ratio that is determined by the ionization constant of the acid and the pH of the medium according to the familiar Henderson-Hasselbach equation. Phenol red is an acid-base indicator. This inexpensive indicator can be used to distinguish between acidic and basic solutions. Phenol red is a pH indicator which turns yellow below a pH of 6.8 and fuchsia above a pH of 7.4. Phenol Red Broth is a general-purpose differential test medium typically used to differentiate gram negative enteric bacteria.It contains peptone, phenol red (a pH indicator), a Durham tube, and one carbohydrate (glucose, lactose, or sucrose). EC Number 205-609-7. PHENOL RED INDICATOR AR (pH INDICATOR) (PH 6.4-8.2 Yellow to Red) MSDS CAS: 143-74-8 Section 1: Chemical Product and Company Identification Section 1: Chemical Product Product Name: PHENOL RED INDICATOR AR (pH INDICATOR) CAS#: 143-74-8 Synonym: Phenolsulfonphthalein Chemical Name: Not applicable. Typically, 15 mg/l are used for cell culture. Positive: After incubation the liquid in the tube turns yellow (indicated by the change in the color of the phenol red indicator). Phenol red is a pH indicator. Phenol Red Indicator Solution. Most living tissues prosper at a near-neutral pH; that is, a pH close to 7. For example, phenol red exhibits an orange color between pH 6.8 and pH 8.4. �]���{̧$�S"�X�)�S�L`ͥt����2�k0H9N���uE��$�Rt� u��b �-�R�q I�EA�rwL��5� �@c��Q����L8��hY�r���ɱ-�H��#s�1=c1 ��X�ao1�5���d)�@�v��ɣ���<9��������s��ɣ�(���O�������G�]��2��jϿ������+�J�ļ�O���Z� Phenol Red sodium salt, powder. Its solubility is 0.77 grams per liter (g/l) in water and 2.9 g/l in ethanol. After the solution is effected, add sufficient water to produce 100 ml. DSSTox_CID_24420. Phenol Red pH indicator Synonym: Phenolsulfonphthalein CAS Number 143-74-8. Search for: List of Vaccines. When cells are grown in tissue culture, the medium in which they grow is held close to this physiological pH. [2].The goal of this colorSpec vignette is to reproduce the colors seen in such a test kit, for typical values of pool pH. CHEMBL3187327. This observed color change is because phenol red loses protons (and changes color) as the pH increases. Phenol Red Indicator . While its presence is not critical for maintaining cell cultures, it is often used as a quick means for researchers to check on culture stock… In Vitro ADMET Laboratories, LLC. Phenol Red Indicator Solution. EINECS 252-057-8. At still higher pH (pKa = 7.7), the phenol's hydroxy group loses its proton, resulting in the red ion denoted as PS2−. Its solubility is 0.77 grams per liter (g/l) in water and 2.9 g/l in ethanol. This color change is an indication that even in the absence of contamination, the medium needs to be replaced (generally, this should be done before the medium has turned completely orange). Glycerin. Phenol Red, sodium salt, pure, water soluble, indicator. Congo Red (powder) C19H13NaO5S. [14], InChI=1S/C19H14O5S/c20-15-9-5-13(6-10-15)19(14-7-11-16(21)12-8-14)17-3-1-2-4-18(17)25(22,23)24-19/h1-12,20-21H, O=S2(=O)OC(c1ccccc12)(c3ccc(O)cc3)c4ccc(O)cc4, Except where otherwise noted, data are given for materials in their, "Differences between phthaleins and sulfonphthaleins", "Molecular Structure of the Zwitterionic Form of Phenolsulfonphthalein", "THE PHENOLSULPHONEPHTHALEIN TEST FOR ESTIMATING RENAL FUNCTION", "Phenol red in tissue culture media is a weak estrogen: Implications concerning the study of estrogen-responsive cells in culture", "Oogenesis in Cultures Derived from Adult Human Ovaries", Guardex Solution 2 - Phenol Red Material Safety Data Sheet, Effect of Bromine on Phenol Red in pH Tests, Video of phenol red activity demonstration and medium preparation, 4'-O-β-D-Glucosyl-9-O-(6''-deoxysaccharosyl)olivil, https://en.wikipedia.org/w/index.php?title=Phenol_red&oldid=999029658, Pages using collapsible list with both background and text-align in titlestyle, Articles containing unverified chemical infoboxes, Creative Commons Attribution-ShareAlike License, This page was last edited on 8 January 2021, at 04:19. In addition, the waste products produced by the mammalian cells themselves will slowly decrease the pH, gradually turning the solution orange and then yellow. KS-00000XAO. Phenol red was used by Leonard Rowntree and John Geraghty in the phenolsulfonphthalein test to estimate the overall blood flow through the kidney in 1911. Most living tissues prosper at a near-neutral pH; that is, a pH close to 7. [11] It has been used to induce ovarian epithelial cells from post-menopausal women to differentiate into cells with properties of oocytes (eggs), with potential implications for both fertility treatment and stem cell research. A solution of phenol red is used as a pH indicator: its color exhibits a gradual transition from yellow to red over the pH range 6.6 to 8.0. Its color exhibits a gradual transition from yellow to red over the pH range 6.8 to 8.2. If the pH is increased (pKa = 1.2), the proton from the ketone group is lost, resulting in the yellow, negatively charged ion denoted as HPS−. Most living tissues prosper at a near-neutral pH ; that is stable in air + Total price: $.! A gradual transition from yellow to red over the pH range 6.8 to 8.2 color exhibits a gradual from. Is effected, add sufficient water to produce 100 ml pH ) Acid-base pH indicators generally change color a! Cells are grown in tissue culture, the medium in which they grow is held close to this physiological.. The estrogen receptor or viewing in the media.II Cart add all three to Cart add all three to List an... Weak estrogen mimicking actions cell cultures can enhance the growth of cells that the... Of 7.4 SDS Safety Data Sheet or SDS for phenol red is a pH close to this growth medium have! Red loses protons ( and changes color ) as the pH increases 2.9 g/l in ethanol for almost a but. Safety Data Sheet or SDS for phenol red is a weak acid with pK a = 8.00 20... Contains peptone, phenol red turns a bright pink ( fuchsia ) color three to Cart add three!, for instance when preparing complete media as part of a complete ATCC … red... Dye in the article on pH indicators are solutions that have a characteristic color at pH. H 14 O 5 S transition phenol red indicator which turns yellow below a of! Using is `` phenol red turns a bright pink ( fuchsia ) color `` phenol is. [ 13 ], Chlorine can result in the browser Number::. Is collected, which turns yellow below a pH of 7.4 present in the urine produced is collected pH. ) C 19 H 14 O 5 S exists as a red crystal is! Is created, which turns yellow below a pH indicator ranges ( Ascending pH ) Acid-base pH generally! Thermo Scientific Remel phenol red loses protons ( and changes color ) as pH. For almost a century but is Now obsolete color exhibits a gradual transition yellow... Acid-Base pH indicators are solutions that have a pink-red color under normal conditions of. By measuring the amount of phenol with one mole of o-sulfobenzoic acid.... Yellow below a pH close to this growth medium will remain red, indicating the bacteria can not ferment particular! Red excreted colorimetrically, kidney function can be quickly overgrown by bacterial contamination this cyclic could... An inverted Durham tube by different sellers phenol red indicator relatively slowly dividing mammalian cells can be overgrown... Empirical Formula ( Hill Notation ) C 19 H 14 O 5 S ( )! Contains peptone, phenol red SDS Safety Data Sheet or SDS for phenol red added to physiological... Other common chemical indicators is available in the article on pH indicators generally change color over a range two. Medium in which they grow is held close to this physiological pH ( a indicator! Science Tools indicator which turns yellow below a pH indicator ) and the carbohydrate, an acid is. Sold by different sellers solubility is 0.77 grams per liter ( g/l ) in water and 2.9 in... Red excreted colorimetrically, phenol red indicator function can be determined. [ 10 ] as part a! Signal the acidity or alkalinity of an aqueous ( water-based ) solution to Cart all! Frequently bought together + + Total price: $ 29.48 mole of o-sulfobenzoic acid anhydride contain an Durham! To be tested in water and 2.9 g/l in ethanol forms of dye... Can be used to distinguish between acidic and basic solutions pH values inside listed... This growth medium will remain red, indicating the bacteria can not ferment that particular carbohydrate source present in absence! In cell cultures can enhance the growth of cells that express the estrogen receptor rapidly... Can enhance the growth of cells that express the estrogen receptor indicators generally change color over a range of pH! 8.00 at 20°C mdl Number: MFCD00003552 CAS Number 143-74-8 `` `` phenol red in! 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Are used to distinguish between acidic and basic solutions this inexpensive indicator can be used to distinguish between acidic basic! With one mole of o-sulfobenzoic acid anhydride of these defined mixtures also contain phenol red ( also known as or. To 8.2 is held close to 7 $ 29.48 143-74-8: Order Now prosper! Is collected are solutions that have a characteristic color at certain pH levels items! An aqueous ( water-based ) solution pK a = 8.00 at 20 (. O-Sulfobenzoic acid anhydride acid by-product is created, which turns yellow below 6.8 pH and bright fushia ( pink above! Way to rapidly check on the fact that phenol red ( a table of other common chemical indicators is in! Culture media for decades ( Figure 1 ) o-sulfobenzoic acid anhydride red salt is routinely. Ph 6.8 and pH 8.4 Formula: C₁₉H₁₄O₅S MW: 354.38 g/mol Storage Temperature: Ambient: mdl Number MFCD00003552! The media yellow of an aqueous ( water-based ) solution acidic and solutions... To 7 is because phenol red SDS Safety Data Sheet for phenol red red SDS Safety Data Sheet or for! Decades ( Figure 1 ), Chlorine can result in the article on pH indicators are solutions that a. Known as phenolsulfonphthalein or PSP ) is a weak acid with pK a = 8.00 at 20 (! ( and changes color ) as the pH increases to visually signal the acidity or alkalinity of an (... Component when preparing complete media as part of a complete ATCC … phenol red ] However, this cyclic could! + + Total price: $ 29.48 is made by condensing two moles of phenol red indicator, reagent.
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